2024-03-28T19:00:26Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/834132022-11-17T02:08:08Zhdl_2115_20042hdl_2115_136Laser-assisted biomineralization on human dentin for tooth surface functionalizationOyane, AyakoSaito, NoriyukiSakamaki, IkukoKoga, KenjiNakamura, MakiNathanael, A. JosephYoshizawa, NorikoShitomi, KanakoMayumi, Kayoko1000050372256Miyaji, Hirofumiopen access© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 InternationalHydroxyapatiteCalcium phosphate (CaP)DentinLaserBiomimetic processCoating497A technique for tooth surface modification with biocompatible calcium phosphate (CaP) has huge potential in dental applications. Recently, we achieved a facile and area-specific CaP coating on artificial materials by a laser-assisted biomimetic process (LAB process), which consists of pulsed laser irradiation in a supersaturated CaP solution. In this study, we induced the rapid biomineralization on the surface of human dentin by using the LAB process. A human dentin substrate was immersed in a supersaturated CaP solution, then its surface was irradiated with weak pulsed laser light for 30 min (LAB process). Ultrastructural analyses revealed that the pristine substrate had a demineralized collagenous layer on its surface due to the previous EDTA surface cleaning. After the LAB process, this collagenous layer disappeared and was replaced with a submicron-thick hydroxyapatite layer. We believe that the laser irradiation induced pseudo-biomineralization through the laser ablation of the collagenous layer, followed by CaP nucleation and growth at the dentin liquid interface. The mineralized layer on the dentin substrate consisted of needle-like hydroxyapatite nanocrystals, whose c-axes were weakly oriented along the direction perpendicular to the substrate surface. This LAB process would offer a new tool enabling tooth surface modification and functionalization through the in situ pseudo-biomineralization.Elsevier2019-12engjournal articleAMhttp://hdl.handle.net/2115/83413https://doi.org/10.1016/j.msec.2019.110061315463610928-4931Materials science and engineering C : materials for biological applications105110061https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/83413/1/Dentin_20191129.pdfapplication/pdf1.67 MB2019-12