2024-03-29T09:34:16Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/837912022-11-17T02:08:08Zhdl_2115_20040hdl_2115_121Type XVII collagen interacts with the aPKC-PAR complex and maintains epidermal cell polarityWatanabe, MikaKosumi, Hideyuki1000000244484Osada, Shin-IchiTakashima, ShotaWang, Yunan1000020443955Nishie, Wataru1000020457055Oikawa, TsukasaHirose, Tomonori1000000146672Shimizu, Hiroshi1000070645457Natsuga, Kenopen accessThis is the peer reviewed version of the following article: Watanabe, M, Kosumi, H, Osada, S‐I, et al. Type XVII collagen interacts with the aPKC‐PAR complex and maintains epidermal cell polarity. Exp Dermatol. 2021; 30: 62– 67., which has been published in final form at https://doi.org/10.1111/exd.14196. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.atypical PKCcell polarityCOL17epidermal stem cellPAR3490Type XVII collagen (COL17) is a transmembrane protein expressed in the basal epidermis. COL17 serves as a niche for epidermal stem cells, and although its reduction has been implicated in altering cell polarity and ageing of the epidermis, it is unknown how COL17 affects epidermal cell polarity. Here, we uncovered COL17 as a binding partner of the aPKC-PAR complex, which is a key regulating factor of cell polarity. Immunoprecipitation-immunoblot assay and protein-protein binding assay revealed that COL17 interacts with aPKC and PAR3. COL17 deficiency or epidermis-specific aPKC lambda deletion destabilized PAR3 distribution in the epidermis, while aPKC zeta knockout did not. Asymmetrical cell division was pronounced in COL17-null neonatal paw epidermis. These results show that COL17 is pivotal for maintaining epidermal cell polarity. Our study highlights the previously unrecognized role of COL17 in the basal keratinocytes.John Wiley & Sons2021-01engjournal articleAMhttp://hdl.handle.net/2115/83791https://doi.org/10.1111/exd.141960906-6705Experimental dermatology3016267https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/83791/1/Watanabe_et_al_HUSCUP.pdfapplication/pdf1.41 MB2021-01https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/83791/2/Watanabe_et_al_HUSCUP_Suppl.%20Fig.pdfapplication/pdf473.17 KB2021-01