2024-03-29T11:51:53Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/838352023-01-20T03:01:20Zhdl_2115_64361hdl_2115_20044hdl_2115_64360hdl_2115_124Insights into phosphatase-activated chemical defense in a marine sponge holobiontJomori, Takahiro1000050812301Matsuda, Kenichi1000050758612Egami, Yoko1000040305496Abe, Ikuro1000050126869Takai, Akira1000070363900Wakimoto, Toshiyukimetadata only access464Marine sponges often contain potent cytotoxic compounds, which in turn evokes the principle question of how marine sponges avoid self-toxicity. In a marine sponge Discodermia calyx, the highly toxic calyculin A is detoxified by the phosphorylation, which is catalyzed by the phosphotransferase CalQ of a producer symbiont, "Candidatus Entotheonella" sp. Here we show the activating mechanism to dephosphorylate the stored phosphocalyculin A protoxin. The phosphatase specific to phosphocalyculin A is CalL, which is also encoded in the calyculin biosynthetic gene cluster. CalL represents a new clade and unprecedently coordinates the heteronuclear metals Cu and Zn. CalL is localized in the periplasmic space of the sponge symbiont, where it is ready for the on-demand production of calyculin A in response to sponge tissue disruption.Royal Society of Chemistry2021-10-06engjournal articleNAhttp://hdl.handle.net/2115/83835https://doi.org/10.1039/d1cb00163a2633-0679RSC Chemical Biology2616001607