2024-03-28T20:12:45Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/842562022-11-17T02:08:08Zhdl_2115_20043hdl_2115_137Downregulation of AlphaB-crystallin in Retinal Pigment Epithelial Cells Exposed to Diabetes-related Stimuli In Vivo and In VitroWu, Di1000060374394Kase, SatoruLiu, YeKanda, Atsuhiro1000050423752Murata, Miyuki1000010245558Ishida, Susumumetadata only accessAlphaB-crystallinretinal pigment epithelial cellsoxidative stresshigh glucosestreptozotocinadvanced glycation end-products490Background/Aim: AlphaB-crystallin plays a pivotal role in many diseases. However, the involvement of alphaB-crystallin in retinal pigment epithelial (RPE) cells with diabetes stimuli remains unknown. The aim of this study is to examine the alterations of RPE cells and alphaB-crystallin expression in diabetic models in vivo and in vitro. Materials and Methods: Diabetic conditions in mice were induced by streptozotocin (STZ). The thickness of the RPE/choroid complex was measured by optical coherence tomography (OCT). Periodic acid-Schiff (PAS) staining was used to investigate the choriocapillaris in histological sections of murine eyeballs and oxidative stress was evaluated using immunofluorescence with anti-4-hydroxynonenal (HNE) antibody. AlphaB-crystallin expression was examined in the RPE/choroid complex using ELISA. Real-Time PCR was performed to evaluate the alphaB-crystallin expression in cultured human RPE cells with high glucose or following advanced glycation end-products (AGE) stimulation. Results: In diabetic mice, OCT-based RPE/choroidal layers were thickened 2 months after STZ stimulation, where PAS-positive dilated choriocapillaris was noted. Immunoreactivity of 4HNE was strongly observed in the RPE layer, from which a significant number of RPE cells was lost. Meanwhile, alphaBcrystallin expression in 2-month STZ mice was significantly lower compared to controls. In accordance with these results, in vitro data showed that the alphaB-crystallin expression was also significantly lower in RPE cells with high glucose or following AGE stimulation compared to untreated cells. Conclusion: In both types of diabetic models the expression of alphaB-crystallin was found to be downregulated in RPE cells and was associated with increased levels of oxidative stress.International Institute of Anticancer Research2022-01engjournal articleNAhttp://hdl.handle.net/2115/84256https://doi.org/10.21873/invivo.126840258-851XIn vivo361132139