2024-03-29T12:22:18Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/853082022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138Cholecystokinin secretion induced by β-conglycinin peptone depends on Gαq-mediated pathways in enteroendocrine cells1000010396301Hira, TohruMaekawa, ToshihiroAsano, Kozo1000070198894Hara, Hiroshiopen accessThe final publication is available at link.springer.comenteroendocrine cellscholecystokinindietary peptideGPCR610Background Intraduodenal administration of peptone prepared from soybean β-conglycinin (BconP) stimulates cholecystokinin (CCK) secretion from enteroendocrine cells, and suppresses food intake in rats. However, the sensing mechanism of BconP by CCK-producing cells is unknown. Aim of the study We investigated signal transduction pathways mediating CCK secretion in response to BconP in the murine CCK-producing cell line, STC-1. Methods STC-1 cells were seeded in 48-well culture plates until sub-confluent and CCK secretion was examined under various conditions. CCK concentration was determined by the enzyme immunoassay. Results BconP dose-dependently induced CCK secretion in STC-1 cells. Treatment with BAPTA-AM, an intracellular Ca2+ chelator, reduced BconP-induced CCK secretion, however, removal of extracellular Ca2+ did not affect the secretory response. Treatment with 2-amino borate (2-APB) reduced CCK releasing responses, suggesting the involvement of IP3. In addition, BconP failed to induce CCK secretion after treatment with the Gαq protein inhibitor (YM-254890). Conclusion These results indicate that Gαq pathway is responsible for BconP-induced CCK secretion in STC-1 cells, and suggest the involvement of a Gαq-coupled GPCR(s) in dietary peptide sensing in enteroendocrine cells.2008engjournal articleAMhttp://hdl.handle.net/2115/85308https://doi.org/10.1007/s00394-008-0764-11436-62071436-6215European Journal of Nutrition482124127https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/85308/2/Eur%20J%20Nutr%2048_124.pdfapplication/pdf283.49 KB2008