2024-03-29T07:02:58Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/853942022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138Soybean β51–63 peptide stimulates cholecystokinin secretion via a calcium-sensing receptor in enteroendocrine STC-1 cellsNakajima, Shingo1000010396301Hira, TohruEto, YuzuruAsano, Kozo1000070198894Hara, Hiroshiopen access(c) 2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalβ51–63 peptideEnteroendocrine cellsCalcium-sensing receptorCholecystokinin610We previously demonstrated that intraduodenal administration of an arginine-rich β51-63 peptide in soybean β-conglycinin suppresses food intake via cholecystokinin (CCK) secretion in rats. However, the cellular mechanisms by which the β51-63 peptide induces CCK secretion remain to be clarified. In the present study, we examined whether the extracellular calcium-sensing receptor (CaR) mediates β51-63-induced CCK secretion in murine CCK-producing enteroendocrine cell line STC-1. CCK secretion and changes in intracellular Ca2+ concentration in response to β51-63 peptide were measured in STC-1 cells under various extracellular Ca2+ concentrations and after treatment with a CaR antagonist. Intracellular Ca2+ concentrations in response to β51-63 peptide and extracellular Ca2+ were also measured in CaR-expressing human embryonic kidney (HEK-293) cells. The β51-63 peptide induced CCK secretion and intracellular Ca2+ mobilization in STC-1 cells under normal (1.2 mM) extracellular Ca2+ conditions in a dose-dependent manner. These responses to β51-63 peptide were reduced by the removal of intra- or extracellular Ca2+ but enhanced by increasing extracellular Ca2+ concentrations. Intracellular Ca2+ mobilization induced by extracellular Ca2+ was also increased by the pretreatment with β51-63 peptide. Treatment with a specific CaR antagonist (NPS2143) inhibited β51-63-induced CCK secretion and intracellular Ca2+ mobilization. In addition, HEK-293 cells transfected with CaR acquired sensitivity to the β51-63 peptide. From these results, we conclude that CaR is the β51-63 peptide sensor responsible for the stimulation of CCK secretion in enteroendocrine STC-1 cells.2010-01-08engjournal articleAMhttp://hdl.handle.net/2115/85394https://doi.org/10.1016/j.regpep.2009.11.0070167-0115Regulatory Peptides1591-3148155https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/85394/1/Regul%20Pept_159_148.pdfapplication/pdf490.09 KB2010-01-08