2024-03-29T08:13:20Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/867662022-11-17T02:08:08Zhdl_2115_20042hdl_2115_136Antiviral effect of cetylpyridinium chloride in mouthwash on SARS-CoV-21000090645095Takeda, Ryo1000030292006Sawa, Hirofumi1000070609403Sasaki, Michihito1000060507169Orba, Yasuko1000000632423Maishi, NakoTsumita, Takuya1000040374558Ushijima, Natsumi1000030399919Hida, Yasuhiro1000090205998Sano, Hidehiko1000000224957Kitagawa, Yoshimasa1000040399952Hida, Kyokometadata only access497Cetylpyridinium chloride (CPC), a quaternary ammonium compound, which is present in mouthwash, is effective against bacteria, fungi, and enveloped viruses. This study was conducted to explore the antiviral effect of CPC on SARS-CoV-2. There are few reports on the effect of CPC against wild-type SARS-CoV-2 at low concentrations such as 0.001%-0.005% (10-50 mu g/mL). Interestingly, we found that low concentrations of CPC suppressed the infectivity of human isolated SARS-CoV-2 strains (Wuhan, Alpha, Beta, and Gamma) even in saliva. Furthermore, we demonstrated that CPC shows anti-SARS-CoV-2 effects without disrupting the virus envelope, using sucrose density analysis and electron microscopic examination. In conclusion, this study provided experimental evidence that CPC may inhibit SARS-CoV-2 infection even at lower concentrations.Nature Portfolio2022-08-18engjournal articleNAhttp://hdl.handle.net/2115/86766https://doi.org/10.1038/s41598-022-18367-6359821182045-2322Scientific reports12114050