2024-03-28T16:59:38Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/875182022-12-20T03:11:51Zhdl_2115_20070hdl_2115_157Novel production of beta-cryptoxanthin in haskap (Lonicera caerulea subsp. edulis) hybrids : Improvement of carotenoid biosynthesis by interspecific hybridizationFujita, Ryohei1000060531845Jin, Shigeki1000000466450Matoba, Kotaro1000050301875Hoshino, Yoichirometadata only accessBlue honeysuckleCarotenoid accumulationFruit qualityLiquid chromatographytandem massspectrometrySmall fruitβ-cryptoxanthinCarotenoids are important color pigments that contribute to human health. Haskap (Lonicera caerulea subsp. edulis), a shrub mainly distributed in Hokkaido, produces violet-blue berries low in carotenoid amount. We have conducted interspecific hybridization between Miyama-uguisukagura (Lonicera gracilipes var. glandulosa) and haskap to obtain interspecific hybrids for improving fruit quality. In this study, we compared four carotenoid concentrations in fruits using liquid chromatography/tandem mass spectrometry (LC/MS/MS) to reveal carot-enoid accumulation changes by interspecific hybridization. beta-Carotene was the main carotenoid, and the con-centration in interspecific hybrids (0.49-0.77 mg/100 g FW) was higher than Miyama-uguisukagura (0.37 mg/ 100 g FW) and haskap (0.25-0.35 mg/100 g FW). beta-Cryptoxanthin concentration was below the quantification limit in haskap strains; however, we could quantify beta-cryptoxanthin in interspecific hybrids. beta-Cryptoxanthin concentration in Miyama-uguisukagura fruits and the interspecific hybrid strain that contained higher beta-cryp-toxanthin tended to increase during maturation, although others did not increase. Our study revealed that interspecific hybridization changed carotenoid biosynthesis, increased beta-carotene, and induced the conversion of beta-cryptoxanthin to haskap. This study proposes a strategy to expand color variation by interspecific hybridization in fruit breeding programs and provides novel materials to analyze the vigor phenomenon in hybrid plants.Elsevier2022-09-29engjournal articleNAhttp://hdl.handle.net/2115/87518https://doi.org/10.1016/j.scienta.2022.1115470304-4238Scientia horticulturae308111547