2024-03-29T00:15:02Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/9322022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138Rapid accumulation of polyphosphate in extraradical hyphae of an arbuscular mycorrhizal fungus as revealed by histochemistry and a polyphosphate kinase/luciferase system1000040273213Ezawa, Tatsuhiro江澤, 辰広Cavagnaro, Timothy R.Smith, Sally E.Smith, F. AndrewOhtomo, Ryoopen accessarbuscular mycorrhizal fungiluciferasemetachromasypolyphosphatepolyphosphate kinaseToluidine Blue O.474.65The definitive version is available at www.blackwellsynergy.comThe rate of polyphosphate accumulation in extraradical hyphae of an arbuscular mycorrhizal fungus was investigated by conventional histochemistry and a new enzymatic method using a bacterial enzyme, polyphosphate kinase. Marigold ( Tagetes patula cv. Bonanza Orange) was inoculated with Archaeospora leptoticha and grown under P-deficient conditions. Extraradical hyphae were harvested at 0, 1, 3 and 24 h after 1 m M P-application. PolyP levels were assessed by both metachromasy of Toluidine blue O and polyphosphate kinase which converted polyP to ATP followed by the ATP-luciferase assay. Percentage of hyphae with metachromatic granules was increased from 25 to 44% from 0 to 1 h, and a maximum of 50% was reach by 3 h. Polyphosphate content was doubled from 1 to 3 h after P-application (4.8–10.0 μmol as Pi mg − 1 protein) at a rate of 46.4 ± 15.1 nmol min − 1 mg − 1 . The rate of polyphosphate accumulation in the hyphae was surprisingly rapid as those of polyphosphate-hyper accumulating microorganisms. The enzymatic method employed in the present study allows highly specific and sensitive assessment of polyphosphate in the mycorrhizal system.Blackwell Publishing2004-02engjournal articleAMhttp://hdl.handle.net/2115/932https://doi.org/10.1046/j.1469-8137.2003.00966.x0028-646X1469-8137New Phytologist1612387392https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/932/1/NP161-2-8137.2003.00966.x.pdfapplication/pdf173.55 KB2004-02