2024-03-29T06:25:36Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/168692022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138The star-type color pattern in Petunia hybrida 'red Star' flowers is induced by sequence-specific degradation of chalcone synthase RNAKoseki, MaikoGoto, KazunoriMasuta, ChikaraKanazawa, Akirachalcone synthaseCucumber mosaic virusPetunia hybridapost-transcriptional gene silencingRNA silencingshort interfering RNA627Petunia hybrida ‘Red Star’ is a variety whose flowers exhibit a star-type red and white bicolor pattern. We analyzed the mRNA levels of six genes involved in anthocyanin biosynthesis. Only the level of chalcone synthase (CHS) mRNA was depressed in the unpigmented flower sectors. Both transcriptional activity and the accumulation of short interfering RNA of CHS in the unpigmented sectors were detected. Viral infection blocked the generation of CHS-silenced sectors. These results indicate that sequence-specific degradation of CHS RNA is the primary cause of the formation of white sectors in ‘Red Star’ flowers.Oxford University PressJournal Articleapplication/pdfhttp://hdl.handle.net/2115/16869https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/16869/1/PCP46-11.pdf0032-07811471-9053Plant and Cell Physiology4611187918832005-11enginfo:pmid/16143597info:doi/10.1093/pcp/pci192This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The definitive publisher-authenticated version Oxford University Press, Plant and Cell Physiology, vol. 46, issue 11, 2005, pp. 1879-1883 is available online at: http://dx.doi.org/10.1093/pcp/pci192 that the author will receive upon publication here.author