2024-03-29T06:35:02Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/170092022-11-17T02:08:08Zhdl_2115_20042hdl_2115_136Preparation and Properties of Immobilized Mercuric Reductase in Porous Glass Carriers多孔質ガラス担体に固定化した水銀還元酵素の作製及びその特性UO, MotohiroNUMATA, MasahikoSUZUKI, MasayasuTAMIYA, EiichiKARUBE, IsaoMAKISHIMA, AkioMercuric reductasePorous glassImmobilized enzymeCarrierActivitypH profileStabilityMercuric ion sensor573The immobilized mercuric reductase covalently coupled to porous glass was prepared and its properties were investigated. Mercuric reductase immobilized on arylamino and carboxyl derivatives exhibited high relative activities about 20-30% compared with soluble enzymes. The optimum pH of mercuric reductase immobilized on these derivatives was 7.5, slightly higher than that of soluble state (7.0-7.2). The stability of mercuric reductase increased with immobilization onto arylamino derivative and the immobilized enzyme maintained at 30℃ for 29 days showed 80-90% of initial activity. As the application of immobilized enzyme, mercuric ion sensor was prepared and it was found that mercuric ion higher than 0.5μM was detectable with this sensor.社団法人 日本セラミックス協会Journal Articleapplication/pdfhttp://hdl.handle.net/2115/17009https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/17009/3/JCSJ100-430.pdf0914-5400Journal of Ceramic Society of Japan1004304331992-04engpublisher