2024-03-28T18:45:06Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/171512022-11-17T02:08:08Zhdl_2115_20040hdl_2115_121Molecular epidemiology of adenoviral conjunctivitis in Hanoi, VietnamJin, Xue-HaiIshiko, HiroakiNguyen, Thanh HaOhguchi, TakeshiAkanuma, MasatakaAoki, KokiOhno, Shigeakiadenovirusconjunctivitismolecular epidemiologyreal-time PCR496Purpose: To investigate the serotypes of adenovirus causing conjunctivitis in Hanoi, Vietnam. Design: Clinical diagnosis of adenoviral conjunctivitis and laboratory-based experimental study. Methods: We collected 21 conjunctival swabs from 21 different patients with a clinical presentation compatible with adenoviral conjunctivitis, in Hanoi, Vietnam. Immunochromatography and real-time polymerase chain reaction (PCR) were performed to detect human adenovirus (HAdV). The sequence of PCR products was analyzed to determine the serotype of HAdV. Results: Of 21 samples, HAdV DNA was detected in 14 samples (66.7%) by real-time PCR. The serotype analysis showed HAdV-8 in 11 samples (78.6%), HAdV-3 in two samples (14.3%), and HAdV-37 in one sample (7.1%). Of 11 HAdV-8 samples, one sample (9.1%) was prototype, and the other 10 samples (90.9%) had identical nucleotide sequence and were identified as a variant of HAdV-8.Elsevier Inc.Journal Articleapplication/pdfhttp://hdl.handle.net/2115/17151https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/17151/1/AJO142-6.pdf0002-9394American Journal of Ophthalmology1426106410662006-12enginfo:pmid/17157595info:doi/10.1016/j.ajo.2006.07.041author