2024-03-29T08:00:24Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/205382022-11-17T02:08:08Zhdl_2115_20044hdl_2115_124Mutagenic effects of 8-hydroxy-dGTP in live mammalian cellsSatou, KazuyaKawai, KazuakiKasai, HiroshiHarashima, HideyoshiKamiya, HiroyukiReactive oxygen species8-Hydroxy-dGTPMutationMammalian cells464The mutagenicity of an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP), was examined using COS-7 cells. 8-OH-dGTP and supF shuttle plasmid DNA were cointroduced by means of cationic liposomes, and the DNAs replicated in the cells were recovered and then transfected into Escherichia coli. 8-OH-dGTP induced A:T->C:G substitution mutations in the COS-7 cells. This result agrees with previous observations indicating that DNA polymerases misincorporate 8-OH-dGTP opposite A in vitro, and that the oxidized deoxyribonucleotide induces A:T->C:G transversions in E. coli. These results constitute the first direct evidence to show that 8-OH-dGTP actually induces mutations in living mammalian cells.ElsevierJournal Articleapplication/pdfhttp://hdl.handle.net/2115/20538https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/20538/1/FRBM.pdf0891-5849Free Radical Biology and Medicine4210155215602007-05-15enginfo:pmid/17448902info:doi/10.1016/j.freeradbiomed.2007.02.024author