2024-03-29T11:21:41Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/251552022-11-17T02:08:08Zhdl_2115_20058hdl_2115_149Suppression of tumor growth by intra-muscular transfer of naked DNA encoding adrenomedullin antagonistMiseki, T.Kawakami, H.Natsuizaka, M.Darmanin, S.Cui, H. Y.Chen, J.Fu, Q.Okada, F.Shindo, M.Higashino, F.Asaka, M.Hamuro, J.Kobayashi, M.hypoxiaglucose deprivationadrenomedullinadrenomedullin antagonistnaked DNA494.5We have recently reported that the intra-tumoral injection of adrenomedullin (AM) antagonist (AMA; AM (22-52)) peptides significantly reduced the in vivo growth of a pancreatic cancer cell line in severely combined immunodeficient (SCID) mice. In the present study, we examined the effects of intra-tumoral and intra-muscular transfers of naked DNA encoding AMA on the in vivo growth of cancer cell lines. We demonstrate that these treatments induce the regression of a pancreatic cancer cell line and a breast cancer cell line inoculated in SCID mice. Furthermore, CD31-positive cells disappear completely from tumor tissues, following treatment, indicating that neo-vascularization is entirely inhibited. These results suggest that the intra-tumoral or intra-muscular transfer of naked DNA encoding AMA might be a promising alternative modality for treating human cancers.Nature Publishing GroupJournal Articleapplication/pdfhttp://hdl.handle.net/2115/25155https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/25155/1/CGT14-1.pdf0929-19030929-1903Cancer Gene Therapy14139442007-01enginfo:pmid/16841081info:doi/10.1038/sj.cgt.7700979Nature Publishing Group, Cancer Gene Therapy, Vol.14-1, 2007, pp. 39-44author