2024-03-29T12:49:31Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/301872022-11-17T02:08:08Zhdl_2115_20040hdl_2115_121Bleeding from the bone marrow enhances remodeling of the in situ frozen-thawed anterior cruciate ligamentYasuda, KazunoriSakai, ToshihikoKondo, EijiOnodera, ShinAnterior cruciate ligamentBone marrowIn situ frozen-thawed treatmentMechanical propertiesReconstruction491.1Background: The purpose of this study is to biomechanically and histologically evaluate the effect of bleeding from bone marrow on remodeling of the in situ frozen-thawed anterior cruciate ligament.
Methods: Forty-four rabbits were used. Eight rabbits were used to evaluate the amount of bleeding at days 1–4. Thirty-six rabbits were divided into two groups, after the right anterior cruciate ligament underwent the freeze-thaw treatment. In Group I, no treatments were applied. In Group II, a tunnel was drilled into the bone marrow the femoral intercondylar notch. Each rabbit was sacrificed at 6 or 12 weeks. We examined the mechanical properties, and the histology of the anterior cruciate ligament. Findings: A small amount of blood clot was observed only on the first day in Group I, while a large amount of blood clot was seen around the anterior cruciate ligament for 3 days after surgery in Group II. In the midsubstance, a number of cells were scattered in Group II, no cells were seen in Group I at 6 weeks. The tangent modulus showed some tendency of difference between Groups I and II at each period. Interpretation:
Bleeding from the bone marrow obviously enhanced extrinsic cell infiltration into the in situ frozen-thawed anterior cruciate ligament at 6 weeks, and showed some effects on its mechanical properties. This study implied that, in anterior cruciate ligament reconstruction, the blood from a bone tunnel play an important role in graft remodeling.Elsevier Ltd.Journal Articleapplication/pdfhttp://hdl.handle.net/2115/30187https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/30187/1/CBM22-8.pdf0268-0033Clinical Biomechanics2289419492007-10enginfo:pmid/17601640info:doi/10.1016/j.clinbiomech.2007.05.007author