2024-03-29T13:28:05Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/301992022-11-17T02:08:08Zhdl_2115_20040hdl_2115_121Comparison of 99mTc-annexin A5 with 18F-FDG for the detection of atherosclerosis in ApoE−/− miceComparison of 99mTc-annexin A5 with 18F-FDG for detecting atherosclerosis in ApoE-/- miceZhao, YanKuge, YujiZhao, SongjiMorita, KoichiInubushi, MasayukiStrauss, H. WilliamBlankenberg, Francis G.Tamaki, NagaraAtherosclerosisApoptosis99mTc-annexin A518F-FDGApolipoprotein E-knockout mouse493.24Purpose: 99mTc-annexin A5, a marker of ongoing apoptosis, and 18F-FDG, a marker of the increased metabolism of inflammatory cells, are supposed to be useful in the detection of metabolically active atheroma. This study reports a comparison of the intralesional distribution of these tracers in relation to lesion development in ApoE−/− mice.
Methods: Male ApoE−/− mice (n = 12–14/group) were maintained on a Western-type diet after the age of 5 weeks. At 25 weeks, 99mTc-annexin A5 or 18F-FDG was injected and the aortas were harvested for autoradiography (ARG) and Oil Red O staining. Regional radioactivity accumulation was compared in relation to the Oil Red O staining score (ranging from 0 to 3, a semiquantitative parameter for evaluating lesion development).
Results: Both 99mTc-annexin A5 and 18F-FDG showed preferential uptake into atherosclerotic lesions, with higher uptake levels for 18F-FDG (mean, 56.07 %ID×kg/m2) than for 99mTc-annexin A5 (mean, 10.38 %ID×kg/m2). The regional uptake levels of each tracer correlated with the Oil Red O staining score (r = 0.65, p < 0.05 for 99mTc-annexin A5; r = 0.56, p < 0.05 for 18F-FDG). The uptake ratios of advanced lesions (score >0.5) to early lesions (score <0.5) were significantly higher for 99mTc-annexin A5 than for 18F-FDG (f = 4.73, p = 0.03).
Conclusion: Both 99mTc-annexin A5 and 18F-FDG accumulate in atherosclerotic lesions and correlate with the severity of each lesion. The higher absolute uptake levels of 18F-FDG may be advantageous for lesion detection, whereas the preferential uptake of 99mTc-annexin A5 in advanced lesions may be a useful indicator of late-stage lesions or vulnerable plaque transformation.Springer Berlin / HeidelbergJournal Articleapplication/pdfhttp://hdl.handle.net/2115/30199https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/30199/1/EJNMMI34-11.pdf0340-69971619-7089European Journal of Nuclear Medicine and Molecular Imaging3411174717552007-11enginfo:pmid/17437104info:doi/10.1007/s00259-007-0433-2The original publication is available at www.springerlink.comauthor