2024-03-29T11:50:10Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/329842022-11-17T02:08:08Zhdl_2115_20070hdl_2115_157Immunological Similarity Between an Olfactory System-Specific Protein and a Testicular Germ Cell Protein in Kokanee Salmon (Oncorhynchus Nerka)Ueda, HiroshiKudo, HideakiShimizu, MunetakaMochida, KazuhikoAdachi, ShinjiYamauchi, Kohei480An olfactory system-specific protein (N24; molecular weight 24 kDa) was examined for immunological similarity to proteins from various organs (heart, intestine, kidney, muscle, ovary and testis) of mature kokanee salmon (Oncorhynchus nerka) by means of Western blotting analysis using a polyclonal antibody to N24. The antibody recognized one 24 kDa protein in the testis but none in the other organs examined. Immunoelectron microscopic analysis revealed that immunoreactive gold particles were mainly concentrated on the nuclei of spermatids and spermatozoa, while no specific gold particles were observed on the nuclei and the cytoplasm of spermatogonia, spermatocytes, Sertoli, Leydig and peritubular cells in maturing and mature testes of kokanee salmon. This is the first description of an antigenic similarity between an olfactory system-specific protein and a testicular germ cell protein in the animal kingdom. This corresponding protein may prove to be a useful molecular marker for studying the mechanism of sperm chemotaxis during fertilization.日本動物学会Journal Articleapplication/pdfhttp://hdl.handle.net/2115/32984https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/32984/1/10_685.pdf0289-0003Zoological Science1046856901993-08eng(c) 日本動物学会 / 本文献の公開は著者の意思に基づくものであるpublisher