2024-03-29T06:26:05Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/389562022-11-17T02:08:08Zhdl_2115_20049hdl_2115_141Pathogenicity of rhabdovirus olivaceus (hirame rhabdovirus; HRV) to hirame and salmonid fishOseko, NorihisaYoshimizu, MamoruKimura, Takahisa663Pathogenicity of the Rhabdovirus olivaceus (hirame rhabdovirus; HRV) to hirame (Japanese flounder) and salmonid fish (rainbow trout, masu, chum, and coho salmon) was studied. Cumulative mortalities of HRV infected hirame reared at 5, 10, 15 and 20 ℃ were 40, 60, 20 and 0 %, respectively. The highest virus titer in mixed samples of kidney and spleen was obtained from fish reared at 5 ℃. Cumulative mortalities of HRV infected rainbow trout, masu, chum, and coho salmon reared at 12 – 14 ℃ were 55, 30, 25 and 5 %, respectively. Mortality of rainbow trout weighing 0.2, 0.5 and 3.0 g was 100, 55 and 40 %, respectively. Mortality of rainbow trout infected with HRV and IHNV was 40 and 100 %, respectively. Histopathologically, in both hirame and rainbow trout, the kidney exhibited necrotic changes such as nuclear degeneration of hematopoietic cells and hemorrhage in the interstitial tissue; the spleen showed necrosis and hemorrhage in the ellipsoid and pulp. In rainbow trout infected with HRV or IHNV similar changes were observed in the kidney and spleen, but in HRV infected fish, no pathological changes were found in the liver of early stages, and necrosis was not observed in the intestine.The Asian Fisheries SocietyConference Paperapplication/pdfhttp://hdl.handle.net/2115/38956https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/38956/1/yoshimizu-82.pdfThe Second Asian Fisheries Forum : proceedings of The Second Asian Fisheries Forum, Tokyo, Japan, 17-22 April 19896396421990engpublisher