2024-03-29T06:59:59Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/425932022-11-17T02:08:08Zhdl_2115_20070hdl_2115_157Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciensGuo, Yang-DongHisano, HiroshiShimamoto, YoshiyaYamada, Toshihikoandrogenic callusanther culturedihaploidGUS activitytransgenic forage grass616Genetic transformation of androgenic-derived amphidiploid Festulolium plants (Lolium perenne L. x Festuca pratensis Huds., 2n=4x=28) by Agrobacterium tumefaciens has been achieved. Anther culture-induced calli of Festulolium "Bx351" were inoculated with Agrobacterium tumefaciens strain LBA4404 carrying pIG121-Hm encoding the hygromycin resistance (hph) and β-glucuronidase (uidA) genes under the control of a CaMV 35S promoter. Twenty-three putative transformants were obtained from the hygromycin selection, 19 of which (82.6%) showed GUS activity. The integration of transgenes was detected by using genomic DNA PCR analysis, RT-PCR analysis and Southern blot hybridization, respectively, which revealed that foreign gene was integrated into the genomes of dihaploid transformants (2n=2x=14). The haploid embryogenic system offers a stable means of transformation, as the introduced trait can be readily fixed through chromosome doubling.SpringerJournal Articleapplication/pdfhttp://hdl.handle.net/2115/42593https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/42593/1/yamada.pdf0167-68571573-5044AA10472996Plant Cell, Tissue and Organ Culture9622192272009-02enginfo:doi/10.1007/s11240-008-9478-6The original publication is available at www.springerlink.comauthor