2024-03-28T21:44:28Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/426952022-11-17T02:08:08Zhdl_2115_20049hdl_2115_141Apoptosis-Inducing Effect of Fucoxanthin on Human Leukemia Cell Line HL-60Hosokawa, MasashiWanezaki, SatoshiMiyauchi, KozueKurihara, HideyukiKohno, HiroyukiKawabata, JunOdashima, ShizuoTakahashi, KoretarofucoxanthinantiproliferationapoptosisDNA fragmentationHL-60 cell498The apoptosis-inducing effect of fucoxanthin on human leukemic HL-60 cells was investigated. Fucoxanthin, which was obtained from the brown alga Undaria pinnatifida, inhibited the proliferation of HL-60 cells and induced DNA fragmentation on agarose gel electrophoresis, a typical characteristic feature of apoptotic cells. The results of sandwich ELISA using anti-biotin-antibody and anti-DNA-antibody also demonstrated DNA fragmentation in accordance with the increase in fucoxanthin concentration and incubation time. Since the level of DNA fragmentation did not increase after 24 h incubation, fucoxanthin appeared to have some effect on cell cycle. In contrast, β-carotene did not show an apoptosis-inducing effect on HL-60 cells. These results suggested that a carotenoid structure might be crucial for inducing apoptosis.Japanese Society for Food Science and TechnologyJournal Articleapplication/pdfhttp://hdl.handle.net/2115/42695https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/42695/1/takahashi_FSTR5_243.pdf1344-66061881-3984AA11320122Food Science and Technology Research532432461999enginfo:doi/10.3136/fstr.5.243publisher