2024-03-28T11:37:14Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/429462022-11-17T02:08:08Zhdl_2115_20039hdl_2115_116Microtubule bundle formation driven by ATP : the effect of concentrations of kinesin, streptavidin and microtubulesKawamura, RyuzoKakugo, AkiraOsada, YoshihitoGong, Jian Ping464Recently, a method was established for the formation of microtubule (MT) assemblies by an active self-organization (AcSO) process, in which MTs were crosslinked during sliding motion on a kinesin-coated surface, and this was coupled with adenosine triphosphate (ATP) hydrolysis. Streptavidin (ST) was the glue used to crosslink biotin-labeled MTs. Although most of the MT assemblies were in the bundle form, they varied in size, shape and motility, depending on the initial conditions used. In this paper, we systematically examined the effects of the concentrations of kinesin, ST and MT on the formation of MT bundles under the initial conditions of the process.IOP PublishingJournal Articleapplication/pdfhttp://hdl.handle.net/2115/42946https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/42946/1/Nano21-14_145603.pdf0957-4484Nanotechnology21141456032010-04-09enginfo:pmid/20215659info:doi/10.1088/0957-4484/21/14/145603This is an author-created, un-copyedited version of an article accepted for publication in Nanotechnology. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The definitive publisher authenticated version is available online at 10.1088/0957-4484/21/14/145603author