2024-03-28T20:38:44Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/431042022-11-17T02:08:08Zhdl_2115_20045hdl_2115_139Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides : Case study on engineered apidaecinsMatsumoto, Ken'ichiroOrikasa, YoshitakeIchinohe, KentaHashimoto, ShigekiOoi, ToshihikoTaguchi, SeiichiCell penetrationFlow cytometryAntibacterial peptideHoney bee463Contributing factors for the antimicrobial activity enhancement of N-terminally engineered mutants of cell-penetrating apidaecins were analyzed based on their cell-penetration efficiency. The flow cytometric analysis of the engineered apidaecins labeled with carboxyfluorescein (FAM) revealed their enhanced cell-penetrating efficiencies into Escherichia coli that should be one of key factors causing the enhanced antimicrobial activity. It is noteworthy that, for one mutant, the enhancement in antimicrobial activity (18-fold higher than wild type) was greater than that of cell penetration (5.9-fold), suggesting that the N-terminal mutation may reinforce both interaction with unidentified intracellular target(s) and cell-penetration efficiency.ElsevierJournal Articleapplication/pdfhttp://hdl.handle.net/2115/43104https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/43104/1/BBRC395-1_7-10.pdf0006-291XBiochemical and Biophysical Research Communications39517102010-04-23enginfo:pmid/20302844info:doi/10.1016/j.bbrc.2010.03.088author