2024-03-29T12:30:27Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/432012022-11-17T02:08:08Zhdl_2115_20044hdl_2115_124Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa CellsIto, ManaSuda, YusukeHarashima, HideyoshiKamiya, Hiroyukireplicating plasmiddeoxynucleoside kinasecell death499To enhance the levels of transgene expression from plasmid-based nonviral vectors, replicating plasmids containing the SV40 origin and the SV40 large T antigen gene, as a model replicating unit, were constructed. The replicating luciferase plasmid DNA produced the luciferase protein more efficiently than the non-replicating luciferase plasmid DNA, as expected. Surprisingly, the introduction of the replicating plasmid DNA containing the Drosophila melanogaster deoxyribonucleoside kinase (Dm-dNK) gene was highly cytotoxic and caused cell death without nucleoside analogs. Our results confirm that transgenes on a replicating plasmid represent an excellent tool for effective protein production and suggest that efficient production of the Dm-dNK protein in tumor cells could be an attractive cancer therapy.Journal Articleapplication/pdfhttp://hdl.handle.net/2115/43201https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/43201/1/33_7_1223_1227.pdf0918-61581347-5215Biological & Pharmaceutical Bulletin337122312272010-07-01enginfo:doi/10.1248/bpb.33.1223publisher