2024-03-28T10:55:44Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/438552022-11-17T02:08:08Zhdl_2115_20042hdl_2115_136Vascular Cell-Like Potential of Undifferentiated Ligament Fibroblasts to Construct Vascular Cell-Specific Marker-Positive Blood Vessel Structures in a PI3K Activation-Dependent MannerOkubo, NaotoIshisaki, AkiraIizuka, TadashiTamura, MasatoKitagawa, YoshimasaThree-dimensional vessel reconstructionAngiogenesisTie-2FLK-1DifferentiationFibroblastsEndothelial cellFibroblast growth factorPI3KPeriodontal ligament491Objective: To evaluate whether fibroblasts derived from periodontal ligament (PDL) retain the ability to differentiate into putative vascular cells and construct vascular cell-specific marker-positive blood vessel structures. We also evaluated the morphological features of the structure and investigated the intracellular molecular mechanism underlying the angiogenic activity of these cells. Methods: Single cell-derived cultures (SCDCs) were established from primary rat ligament fibroblast cultures, and their expression of ligament cell-, mesenchymal stem cell (MSC)-, and vascular cell-specific markers was evaluated by RT-PCR and immunocytochemistry. The ability of the cells to construct a blood vessel structure was evaluated in a three-dimensional type I collagen scaffold. The morphological and immunohistological characteristics of the structure were then evaluated. Results: Each SCDC expressed endothelial cell (EC) and smooth muscle cell-specific markers, in addition to MSC- and ligament cell-specific markers. SCDC2 cells, which abundantly expressed the EC markers Flk-1 and Tie-2, vigorously constructed a blood vessel structure in a phosphoinositide 3-kinase activation-dependent manner. Conclusion: PDL fibroblasts have the potential to construct an EC marker-positive blood vessel-like structure. Consequently, the fibroblastic lineage in ligament tissue could be a candidate precursor for construction of a vascular system around damaged ligament tissue to facilitate its regeneration.KargerJournal Articleapplication/pdfhttp://hdl.handle.net/2115/43855https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/43855/1/JVR47-5_369-383.pdf1018-1172Journal of Vascular Research4753693832010-08enginfo:pmid/20110728info:doi/10.1159/000277724Copyright © 2010 S. Karger AG, Baselauthor