2024-03-29T13:25:42Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/449612022-11-17T02:08:08Zhdl_2115_20070hdl_2115_157Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackeniiHirano, TomonariHoshino, YoichiroCyrtanthus mackeniimale germ unitmicrotubulepollen tubesperm dimorphism627Pollen tubes of Cyrtanthus mackenii, a species with bicellular pollen, were cultured in vitro to investigate nuclear phase changes during generative cell division and male germ unit (MGU) formation using flow cytometric analysis. Results revealed that sperm cells were formed after 12 h of culture. During sperm maturation, the nuclei of sperm cells were not associated with the vegetative nucleus (unassociated sperm cells; Sua) and became longer than those of sperm cells associated with the vegetative nucleus (Svn). These findings indicate that the pair of sperm cells in the C. mackenii MGU is dimorphic in terms of nuclear shape. Dimorphism coincides with anti-α-tubulin antibody immunofluorescence, which was higher in the Sua than in Svn. Following treatment with oryzalin, triggering microtubule depolymerization, differences between nuclear shape in the two sperm nuclei disappeared, suggesting that microtubule accumulation between sperm cells in the MGU correlates with differences in the nuclear shape.SpringerJournal Articleapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/2115/44961https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/44961/6/497_2009_123_MOESM1_ESM.pdfhttps://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/44961/7/497_2009_123_MOESM2_ESM.pdfhttps://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/44961/5/hoshino_SPR2010.pdf0934-0882Sexual Plant Reproduction2321531622010-03enginfo:pmid/20490968info:doi/10.1007/s00497-009-0123-2The original publication is available at www.springerlink.comauthor