2024-03-29T00:42:04Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/455332022-11-17T02:08:08Zhdl_2115_20051hdl_2115_144The Escherichia coli highly expressed entD gene complements the pfaE deficiency in a pfa gene clone responsible for the biosynthesis of long-chain n-3 polyunsaturated fatty acidsSugihara, ShinjiOrikasa, YoshitakeOkuyama, HidetoshiASKA cloneEntDeicosapentaenoic acidpfaEphosphopantetheinyl transferasepolyunsaturated fatty acid465The Escherichia coli entD gene, which encodes an Sfp-type phosphopantetheinyl transferase (PPTase) that is involved in the biosynthesis of siderophore, is available as a high-expression ASKA clone (pCA24N::entD) constructed from the E. coli K-12 strain AG1. In E. coli DH5α, pCA24N::entD complemented a pfaE-deficient clone that comprised pfaA, pfaB, pfaC and pfaD, which are four of the five pfa genes that are responsible for the biosynthesis of eicosapentaenoic acid derived from Shewanella pneumatophori SCRC-2738. Sfp-type PPTases are classified into the EntD and PfaE groups, based on differences between their N-terminal-domain structures. Here, we showed that all Sfp-type PPTases may have the potential to promote the biosynthesis of long-chain n-3 polyunsaturated fatty acids (n-3 PUFAs).Blackwell PublishingJournal Articleapplication/pdfhttp://hdl.handle.net/2115/45533https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/45533/1/FEMSML307-2_207-211.pdf0378-1097FEMS Microbiology Letters30722072112010-06enginfo:doi/10.1111/j.1574-6968.2010.01987.xThe definitive version is available at www.blackwell-synergy.comauthor