2024-03-29T14:50:09Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/471672023-10-11T01:55:57Zhdl_2115_20049hdl_2115_141Occurrence of all-cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3) in roughscale sole Clidoderma asperrimum flesh lipidsサメガレイClidoderma asperrimum の筋肉脂質における全-cis-5,8,11,14,17,20,23-ヘキサコサヘプタエン酸(26:7n-3)の存在Fukuda, YuukiAndo, YasuhiroHexacosaheptaenoic acidVery long chain polyunsaturated fatty acidFatty acidRoughscale soleClidoderma asperrimumGCGC–MS464Fatty acid analysis of roughscale sole Clidoderma asperrimum flesh lipids was carried out by gas chromatography. An unidentified peak appeared in the chromatogram in the elution region of ≥C24 fatty acids. After enrichment by solvent partitioning, reversed-phase thin-layer chromatography (TLC), and argentation TLC, the peak component was subjected to structural analyses. The partially hydrogenated products after reaction with hydrazine hydrate gave seven isomers of cis-hexacosenoic acid (26:1). Gas chromatography-mass spectrometry (GC–MS) analyses of their dimethyl disulfide adducts identified the monounsaturates as 5-, 8-, 11-, 14-, 17-, 20-, and 23-26:1. The peak component was assigned to all-cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3). GC–MS analyses of the 4,4-dimethyloxazoline derivative and methyl ester confirmed this structure. This fatty acid is a rare, very long-chain polyunsaturated fatty acid (VLCPUFA). The concentrations of the acid found in roughscale sole were 0.69 ± 0.34% (N = 5) of total fatty acids in flesh lipids. Roughscale sole appears to be characterized by the occurrence of 26:7n-3, which is lacking in popular sources of methylene-interrupted VLCPUFA, such as vertebrate retina, spermatozoa, and herring. 北海道産サメガレイの筋肉脂質の脂肪酸をGC で分析したところ,炭素数24 以上の領域に未知のピークが出現した。この成分を濃縮後,数種の誘導体に変換してGC-MSに供した結果,同成分は全-cis-5,8,11,14,17,20,23-ヘキサコサヘプタエン酸(26:7n-3)と同定された。総脂肪酸中の含有量は供試5 個体の平均で0.69±0.34%であった。サメガレイは,n-3 系およびn-6 系超長鎖ポリエン酸の給源として一般的な高等動物の網膜,精子,ニシン油などには見られない26:7n-3 を含む点で特徴的である。Japanese Society of Fisheries ScienceJournal Articleapplication/pdfhttp://hdl.handle.net/2115/47167https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/47167/1/Fukuda%26Ando.pdf0919-92681444-2906AA10993718Fisheries Science7758758822011-09enginfo:doi/10.1007/s12562-011-0387-y© 2011 公益社団法人日本水産学会author