2024-03-29T16:00:39Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/473962023-09-29T02:41:37Zhdl_2115_20051hdl_2115_144Effects of γ-hexachlorocyclohexane on apoptosis induced by serum deprivation in PC12 cellsAoki, KentaroEgawa, MariSaito, TakeshiHosokawa, ToshiyukiKurasaki, MasaakiApoptosisγ-HCHPC12 cellcaspasecytochrome CBcl-2 family463The effects of γ-hexachlorocyclohexane, one of the endocrine disrupters, widely used for agricultural and medicinal purpose, on apoptosis in PC12 cells were investigated using western blotting analysis and reverse transcriptase-DNA polymerase chain reaction (RT-PCR) method. Apoptosis is a fundamental process necessary for development of individuals and organs. Although γ -HCH at high concentration did not have an effect on cell viability and apoptosis, DNA fragmentation was slightly enhanced, and apoptotic factors; Bax, Bad, cytochrome c and caspase-3 showed tendency to increase by the addition of a low dose of γ-HCH to the cell medium. However these changes were not statistically significant. It was concluded that γ -HCH did not affect on apoptosis in the PC12 cell line system, although γ -HCH has been reported to induce apoptosis in other cell lines.Taylor & FrancisJournal Articleapplication/pdfhttp://hdl.handle.net/2115/47396https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/47396/1/AokiJESH3.pdf0360-12341532-4109AA00697424Journal of Environmental Science and Health, Part B : Pesticides, Food Contaminants, & Agricultural Wastes4364714752008-08enginfo:pmid/18665982info:doi/10.1080/03601230802174573This is a preprint of an article submitted for consideration in the Journal of Environmental Science and Health, Part B © 2008 Taylor & Francis. Journal of Environmental Science and Health, Part B is available online at: www.tandfonline.com.author