2024-03-29T09:11:11Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/486432022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138An apple atp9 pseudogene is maintained at high copy number in 'Golden Delicious'-type mitochondria but is present substoichiometrically in 'Delicious'-type mitochondriaKato, SumieKitazaki, KazuyoshiWakatsuki, AkihitoKishima, YujiMikami, TetsuoAppleCytoplasmic diversityMitochondrial atp9 geneRecombinationSubstoichiometric sequences467In this study, the mitochondrial atp9 gene sequence of an apple cultivar 'Golden Delicious' was found to exist in one intact version and two truncated versions (termed φatp9-1 and φatp9-2). Interestingly, the φatp9-1 sequence is maintained at high copy number in the six 'Golden Delicious'-cytotype cultivars examined but present substoichiometrically in eight 'Delicious'-cytotype cultivars. Our data also suggest that φatp9-1 originated in a homologous recombination event mediated by the short repeat in a common ancestral mitochondrial genome of 'Golden Delicious' and 'Delicious', and was preferentially amplified in an evolutionary lineage that gave rise to the 'Golden Delicious'-type genome. On the other hand, φatp9-2 was revealed to be present in high abundance in all 14 cultivars examined.Elsevier B.V.Journal Articleapplication/pdfhttp://hdl.handle.net/2115/48643https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/48643/1/SH134_237-240.pdf0304-4238Scientia Horticulturae1342372402012-02-01enginfo:doi/10.1016/j.scienta.2011.10.030author