2024-03-28T17:21:30Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/487222022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A. majusKim, Bo MinInaba, Jun-ichiMasuta, ChikaraCMVfunctional genomicsVIGS615The Arabidopsis gene AINTEGUMENTA (At-ANT) functions in cell proliferation and organ growth. The ANT protein has two copies of the AP2 domains, R1 and R2. Recently, a partial cDNA sequence of the At-ANT homolog in Antirrhinum majus (Am-ANT) was reported (Delgado-Benarroch et al., 2009). Here, we used virus-induced gene silencing (VIGS) to analyze the function of the reported Am-ANT. We then determine the open reading frame (ORF) of Am-ANT and its predicted amino acid sequence. We induced VIGS using Cucumber mosaic virus (CMV-A1) and suppressed the level of Am-ANT mRNA and noted any phenotypic changes. The function of Am-ANT was very similar to that of At-ANT. The A1:ANT-infected Antirrhinum plants had smaller floral organs and leaves, even though cell sizes were unchanged in flowers and larger in leaves. The CMV-based VIGS showed that the cloned Am-ANT gene was indeed functional in cell proliferation and organ growth as observed for At-ANT. In A. majus, CMV vector provide great advantages for analysis of gene functions.Korean Society for Horticultural Science in co-publication with SpringerJournal Articleapplication/pdfhttp://hdl.handle.net/2115/48722https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/48722/1/HEB52-2_176-182.pdf2211-3452Horticulture, Environment, and Biotechnology5221761822011-04enginfo:doi/10.1007/s13580-011-0172-yThe original publication is available at www.springerlink.comauthor