2024-03-29T14:53:05Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/520812022-11-17T02:08:08Zhdl_2115_20049hdl_2115_141ホスホリパーゼA2による高度不飽和脂肪酸含有ホスファチジルコリンの合成と細胞分化誘導作用に及ぼす影響Synthesis of Phosphatidylcholine Containing Highly Unsaturated Fatty Acid by Phospholipase A2 and Effect on Retinoic Acid Induced Differentiation of HL-60 Cells細川, 雅史大島, 宏哲甲野, 裕之高橋, 是太郎羽田野, 六男小田島, 粛夫660Phosphatidylcholine containing highly unsaturated fatty acid (HUFA-PC) was prepared by porcine pancreatic phospholipase A2, which catalyzed esterification between lysophosphatidylcholine (LPC) and highly unsaturated fatty acid (HUFA), under a scaled-up reaction system. Fatty acid mixture prepared from sardine oil, purified eicosapentaenoic acid (EPA), and purified docosahexaenoic acid (DHA) were used as the substrates of HUFA. The yield of HUFA-PC was 17.0-19.9%. Synthesized phosphatidylcholine containing DHA (DHA-PC) prepared from LPC and DHA had a DHA content of 46.5%, which indicated almost the maximum theoretical incorporation rate. DHA-PC molecular species consisted of (16:0, DHA), (18:0, DHA), (18:1, DHA), (18:2, DHA), and (18:3, DHA). It was obvious that the acyl groups in sn-1 of these molecular species originated from the substrate LPC. The rate of retinoic acid induced differentiation of the human promyelocytic leukemia cell line (HL-60) was promoted by growing HL-60 cells in 10% FBS-PNM pre-treated with either EPA-PC or DHA-PC.日本水産学会Journal Articleapplication/pdfhttp://hdl.handle.net/2115/52081https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/52081/1/JSFS59_309.pdf0021-53921349-998X日本水産学会誌5923093141993jpninfo:doi/10.2331/suisan.59.309© 1993 公益社団法人日本水産学会publisher