2024-03-28T14:50:39Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/521372022-11-17T02:08:08Zhdl_2115_20044hdl_2115_124Enhanced transgene expression from chromatinized plasmid DNA in mouse liverKamiya, HiroyukiMiyamoto, ShihoGoto, HitomiKanda, Genki N.Kobayashi, MiwakoMatsuoka, IchiroHarashima, HideyoshiExogenous DNAHistoneNucleosomeIntranuclear dispositionExpression efficiency499Plasmid DNA was chromatinized with core histones (H2A, H2B, H3, and H4) in vitro and was delivered into mouse liver by hydrodynamics-based administration. Transgene expression from the chromatinized plasmid DNA was more efficient than that from plasmid DNA delivered in the naked form. The use of acetylation-enriched histones isolated from cells treated with a histone deacetylase inhibitor (trichostatin A) seemed to be more effective. These results indicated that chromatinized plasmid DNA is useful for efficient transgene expression in vivo.Elsevier B.V.Journal Articleapplication/pdfhttp://hdl.handle.net/2115/52137https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/52137/1/IJP441-1-2_146-150.pdf0378-5173International Journal of Pharmaceutics4411-21461502013-01-30enginfo:doi/10.1016/j.ijpharm.2012.12.004author