2024-03-29T02:08:23Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/529442022-11-17T02:08:08Zhdl_2115_20053hdl_2115_145Nanoscale fluctuations on epithelial cell surfaces investigated by scanning ion conductance microscopyMizutani, YusukeChoi, Myung-HoonCho, Sang-JoonOkajima, Takaharubiological techniquesbiomechanicsbiomembrane transportcellular biophysicsion microscopes423Nanoscale fluctuations on the apical surfaces of epithelial cells connected to neighboring cells were investigated by scanning ion conductance microscopy. Mapping the ion current as a function of the tip-surface distance revealed that in untreated cells, the apparent fluctuation amplitude increased towards the cell center. We found that the spatial dependence was less correlated with the heterogeneities of cell stiffness but was significantly reduced when actin filaments were disrupted. The results indicate that apical surface fluctuations are highly constrained at the cell-cell interface, in the vertical direction to the surface and by the underlying actin filaments. (C) 2013 AIP Publishing LLC.AIPJournal Articleapplication/pdfhttp://hdl.handle.net/2115/52944https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/52944/1/ApplPhysLett_102_173703.pdf0003-69511077-3118AA00543431Applied Physics Letters10217173703-1173703-42013-04-29enginfo:doi/10.1063/1.4803469Copyright 2013 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Appl. Phys. Lett. 102, 173703 (2013) and may be found at https://dx.doi.org/10.1063/1.4803469publisher