2024-03-28T20:53:34Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/531432022-11-17T02:08:08Zhdl_2115_20049hdl_2115_141Aeromonas salmonicidaの自発凝集性に及ぼす分離培養温度の影響Effect of incubation temperature for isolation on auto-agglutination of Aeromonas salmonicida茂木(戸田), 省三野村, 哲一吉水, 守Aeromonas salmonicida auto-agglutinationFurunculosisIncubation temperatureTrout663ヤマメせっそう病魚から 15, 20, 25℃ の3温度で分離培養した A. salmonicida を同じ温度で継代培養し, その自発凝集性を観察した。15℃及び20℃の場合は明瞭な自発凝集性を示したが, 25℃の場合は自発凝集性が弱まった。イワナせっそう病魚から20℃で分離培養した A. salmonicida も25℃で継代培養すると同様の傾向が観察され, A. salmonicida の分離培養は20℃あるいはそれ以下で行うべきであると考えられた。Aeromonas salmonicida was isolated from diseased yamame (Oncorhynchus masou) at 15, 20, and 25°C. Ten colonies were taken from each of ten isolates and were subcultured at the corresponding temperatures. Colonies incubated at 15 and 20°C showed strong auto-agglutination but 40% of colonies incubated at 25°C didn't clearly show auto-agglutination. It was also observed that strains isolated from diseased iwana (Salvelinus leucomaenis) at 20°C and subcultured at 25°C showed weak or no agglutination. These observations strongly suggested that A. salmonicida should be isolated and subcultured at 15°C or 20°C in order to retain their auto-agglutinability.日本魚病学会Journal Articleapplication/pdfhttp://hdl.handle.net/2115/53143https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/53143/1/article75.pdf0388-788X1881-7335AN00063165魚病研究30167681995-03-15jpninfo:doi/10.3147/jsfp.30.67publisher