2024-03-28T09:13:59Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/576612022-11-17T02:08:08Zhdl_2115_20056hdl_2115_147Vertical distribution of major sulfate-reducing bacteria in a shallow eutrophic meromictic lakeKubo, KyokoKojima, HisayaFukui, ManabuMeromictic lakeSulfate-reducing bacteriaaprASulfide465The vertical distribution of sulfate-reducing bacteria was investigated in a shallow, eutrophic, meromictic lake, Lake Harutori, located in a residential area of Kushiro, Japan. A steep chemocline, characterized by gradients of oxygen, sulfide and salinity, was found at a depth of 3.5-4.0 m. The sulfide concentration at the bottom of the lake was high (up to a concentration of 10.7 mM). Clone libraries were constructed using the aprA gene, which encodes adenosine-5'-phosphosulfate reductase subunit A, in order to monitor sulfate-reducing bacteria. In the aprA clone libraries, the most abundant sequences were those from the Desulfosarcina-Desulfococcus (DSS) group. A primer set for a DSS group-specific 16S rRNA gene was used to construct another clone library, analysis of which revealed that the uncultured group of sulfate-reducing bacteria, SEEP SRB-1, accounted for nearly half of the obtained sequences. Quantification of the major bacterial groups by catalyzed reporter deposition-fluorescence in situ hybridization demonstrated that the DSS group accounted for 3.2-4.8% of the total bacterial community below the chemocline. The results suggested that the DSS group was one of the major groups of sulfate-reducing bacteria and that these presumably metabolically versatile bacteria might play an important role in sulfur cycling in Lake Harutori. (C) 2014 Elsevier GmbH. All rights reserved.ElsevierJournal Articleapplication/pdfapplication/pdfhttp://hdl.handle.net/2115/57661https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/57661/1/manuscript.pdfhttps://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/57661/2/suppl.pdf0723-2020Systematic and Applied Microbiology3775105192014-10enginfo:pmid/25034383info:doi/10.1016/j.syapm.2014.05.008author