2024-03-28T09:52:40Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/582642022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138Transcriptional Wiring for Establishing Cell Lineage Specification at the Blastocyst Stage in CattleNagatomo, H.Kagawa, S.Kishi, Y.Takuma, T.Sada, A.Yamanaka, K.-i.Abe, Y.Wada, Y.Takahashi, M.Kono, T.Kawahara, M.bovineblastocystcell lineage specificationgene expression640Mice and cattle use distinct pathways for the first cell segregation into inner cell mass (ICM) and trophectoderm (TE) lineages at the blastocyst stage. However, limited knowledge is available regarding reliable transcriptional networks that orchestrate complex developmental processes at this stage in nonrodent species. In order to elucidate the site-dominant transcriptomic properties of bovine blastocysts, we separated cell samples into the ICM and TE using both mechanical and chemical methods and performed in silico prescreening for candidate genes that were site-dominantly expressed in bovine blastocysts. We further performed quantitative RT-PCR and in situ hybridization using the site-specific cell samples. As a result, we identified 7 ICM-dominant genes and 5 TE-dominant genes not yet found in earlier studies. Our findings provide novel insights into the mechanism of cell-fate specification in the preimplantation bovine embryo.the Society for the Study of ReproductionJournal Articleapplication/pdfhttp://hdl.handle.net/2115/58264https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/58264/1/1%20BOR.pdf0006-33631529-7268Biology of Reproduction8861582013-01-01enginfo:pmid/23677984info:doi/10.1095/biolreprod.113.108993author