2024-03-29T02:34:05Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/593252022-11-17T02:08:08Zhdl_2115_20057hdl_2115_148Multi-point Scanning Two-photon Excitation Microscopy by Utilizing a High-peak-power 1042-nm LaserOtomo, KoheiHibi, TerumasaMurata, TakashiWatanabe, HirotakaKawakami, RyosukeNakayama, HiroshiHasebe, MitsuyasuNemoto, TomomiTwo-photon excitation microscopymulti-point laser scanning methodconfocal microscopyYb-based laser433The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam's scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.Japan Society for Analytical ChemistryJournal Articleapplication/pdfhttp://hdl.handle.net/2115/59325https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/59325/1/31_307-1.pdf0910-6340Analytical sciences3143073132015-04-10enginfo:pmid/25864674info:doi/10.2116/analsci.31.307publisher