2024-03-29T09:35:11Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/59332022-11-17T02:08:08Zhdl_2115_20053hdl_2115_145Nitric Oxide Produced During Ischemia Is Toxic but Crucial to Preconditioning-Induced Ischemic Tolerance of Neurons in CultureKawahara, KoichiYanoma, JunjiTanaka, MotokiNakajima, TakayukiKosugi, TatsuroIschemiapreconditioningnitric oxideneurotoxicitycortical culture491.37The present study investigated the roles of nitric oxide (NO) in preconditioning (PC)-induced neuronal ischemic tolerance in cortical cultures. Ischemia in vitro was simulated by subjecting cultures to both oxygen and glucose deprivation (OGD). A sublethal OGD (PC) significantly increased the survival rate of neurons when cultures were exposed to a lethal OGD 24 h later. Both the inhibition of nitric oxide synthase (NOS) and scavenging of NO during PC significantly attenuated the PC-induced neuronal tolerance. In addition, exposure to an NO donor emulated the PC. In contrast, the inhibition of NOS and the scavenging of NO during lethal OGD tended to increase the survival rate of neurons. This study suggested that NO produced during ischemia was fundamentally toxic, but critical to the development of PC-induced neuronal tolerance.Springer NetherlandsJournal Articleapplication/pdfhttp://hdl.handle.net/2115/5933https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/5933/1/NR29-4.pdf0364-3190Neurochemical Research2947978042004-04enginfo:doi/10.1023/B:NERE.0000018853.30131.4dThe original publication is available at www.springerlink.com.
Former publisher: Kluwer Academic Publishers.author