2024-03-29T15:34:06Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/598472022-11-17T02:08:08Zhdl_2115_20076hdl_2115_597Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D.Sasaki, MichihitoHasebe, RieMakino, YoshinoriSuzuki, TadakiFukushi, HidetoOkamoto, MinoruMatsuda, KazuyaTaniyama, HiroyukiSawa, HirofumiKimura, Takashi649The endotheliotropism of equine herpesvirus-1 (EHV-1) leads to encephalomyelitis secondary to vasculitis and thrombosis in the infected horse central nervous system (CNS). To identify the host factors involved in EHV-1 infection of CNS endothelial cells, we performed functional cloning using an equine brain microvascular endothelial cell cDNA library. Exogenous expression of equine major histocompatibility complex (MHC) class I heavy chain genes conferred susceptibility to EHV-1 infection in mouse NIH3T3 cells, which are not naturally susceptible to EHV-1 infection. Equine MHC class I molecules bound to EHV-1 glycoprotein D (gD), and both anti-gD antibodies and a soluble form of gD blocked viral entry into NIH3T3 cells stably expressing the equine MHC class I heavy chain gene (3T3-A68 cells). Treatment with an anti-equine MHC class I monoclonal antibody blocked EHV-1 entry into 3T3-A68 cells, equine dermis (E. Derm) cells and equine brain microvascular endothelial cells. In addition, inhibition of cell surface expression of MHC class I molecules in E. Derm cells drastically reduced their susceptibility to EHV-1 infection. These results suggest that equine MHC class I is a functional gD receptor that plays a pivotal role in EHV-1 entry into equine cells.Wiley-BlackwellJournal Articleapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/2115/59847https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/59847/4/GTC_1491_sm_FigureS1-S2.pdfhttps://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/59847/3/GTC_1491_sm_TableS1.pdfhttps://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/59847/2/GTC_1491_sm_TableS2.pdfhttps://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/59847/5/Sasaki%202011%20Genes%20Cells.pdf1356-95971365-2443AA11078945Genes to Cells1643433572011-04enginfo:pmid/21306483info:doi/10.1111/j.1365-2443.2011.01491.xhttps://creativecommons.org/licenses/by-nc-sa/4.0/deed.japublisher