2024-03-29T15:08:26Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/598732022-11-17T02:08:08Zhdl_2115_20042hdl_2115_136Influence of Root Dentin Surface Conditioning with Bone Morphogenetic Protein-2 on Periodontal Wound Healing in Beagle DogsMIYAJI, HirofumiSUGAYA, TsutomuIBE, KeisukeISHIZUKA, RyousukeTOKUNAGA, KeisukeKAWANAMI, Masamitsubone morphogenetic protein-2root dentin surface conditioningperiodontal wound healingdog497Bone morphogenetic protein-2 (BMP-2) may play a major role in osteoinduction and osteoclast differentiation. The influence of BMP-2 conditioning in graded doses to root dentin surface on periodontal wound healing was examined.Twenty-four periodontal defects were surgically created on the buccal roots of four beagle dogs. The denuded root dentin surfaces were demineralized with 24% EDTA, 0.1 and 1.0 μg/μL BMP-2 solution (15 μL) was applied, and the groups were labeled as low-dose BMP (BL) and high-dose BMP (BH), respectively. In the control roots, phosphate-buffered saline was applied to the root surface. Specimens were histologically analyzed 16 weeks after surgery.Periodontal wound healing was stimulated by conditioning with BMP-2. Formation of alveolar bone and cementum-like tissue was observed; however, in BH group, ankylosis and root resorption were accelerated in the defect area, and there was little evidence of periodontal ligament formation between alveolar bone and root surface.Japanese Association of Regenerative Dentistry日本再生歯科医学会Journal Articleapplication/pdfhttp://hdl.handle.net/2115/59873https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/59873/1/2011%20miyaji%20J%20Oral%20Tissue%20Engin.pdf1348-96231880-0823AA1195240XJournal of Oral Tissue Engineering831731802011-03-30enginfo:doi/10.11223/jarde.8.173130004456618日本再生歯科医学会(利用は著作権の範囲内に限られます)publisher