2024-03-28T19:10:22Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/627122022-11-17T02:08:08Zhdl_2115_20056hdl_2115_147Direct interaction with ACR11 is necessary for post-transcriptional control of GLU1-encoded ferredoxin-dependent glutamate synthase in leavesTakabayashi, AtsushiNiwata, AkihiroTanaka, AyumiBecause it plays an essential role in nitrogen (N) assimilation and photorespiration, the glutamine synthetase (GS)/glutamate synthase (GOGAT) system is widely accepted as occupying a central position in leaf N metabolism. However, the regulation of GOGAT at the post-transcriptional level is poorly understood. Here, we show that ACR11, an ACT (acronym for aspartate kinase, chorismate mutase, and TyrA) domain-containing family protein, interacts with Glu1-encoded ferredoxin (Fd)-GOGAT in Arabidopsis chloroplasts. In addition, Arabidopsis acr11 mutants have lost the capability to control Fd-GOGAT levels in response to light/dark diurnal cycles, nitrogen inputs, and changes in photorespiratory activity. Considering that ACR11 has putative glutamine-binding domains, our results indicate that ACR11 is necessary for post-transcriptional control of leaf Glu1-encoded Fd-GOGAT. This regulation takes place through direct interaction of ACR11 and Fd-GOGAT, possibly in an allosteric manner.Nature Publishing GroupJournal Articleapplication/pdfhttp://hdl.handle.net/2115/62712https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/62712/1/srep29668.pdf2045-2322Scientific reports6296682016-07-14enginfo:doi/10.1038/srep29668https://creativecommons.org/licenses/by/4.0/publisher