2024-03-29T00:01:01Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/631782022-11-17T02:08:08Zhdl_2115_20045hdl_2115_139Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductaseYokoo, ToshinoriMatsumoto, Ken'ichiroOoba, TakashiMorimoto, KenjiroTaguchi, Seiichipolyhydroxyalkanoatepolyhydroxybutyratebiodegradable plastic579Highly active mutant of NADPH-dependent acetoacetyl-CoA reductase (PhaB) was expressed in Nicotiana tabacum cv. Bright Yellow-2 cultured cells to produce poly(3-hydroxybutyrate) [P(3HB)]. The mutated PhaB increased P(3HB) content by three-fold over the control, indicating that the mutant was a versatile tool for P(3HB) production. Additionally, the PhaB-catalyzed reaction was suggested to be a rate-limiting step of P(3HB) biosynthesis in tobacco BY-2 cells.Journal Articleapplication/pdfhttp://hdl.handle.net/2115/63178https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/63178/1/acceptedmanuscript.pdf0916-84511347-6947Bioscience, Biotechnology, and Biochemistry7969869882015-06enginfo:pmid/25647430info:doi/10.1080/09168451.2014.1002448This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on June 2015, available online: http://wwww.tandfonline.com/10.1080/09168451.2014.1002448.author