2024-03-29T07:16:39Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/632062022-11-17T02:08:08Zhdl_2115_20045hdl_2115_1393,3 ',5,5 '-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based AssaysBusa, Lori Shayne AlamoKomatsu, TakeshiMohammadi, SaeedMaeki, MasatoshiIshida, AkihikoTani, HirofumiTokeshi, ManabuTMB oxidationhorseradish peroxidasepaper-based analytical devicesphotolithographywax-printing430We report on the colorimetric oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide using horseradish peroxidase on photolithography-fabricated (P-PAD) and wax-printed (W-PAD) paper-based analytical devices. Fabricating PADs via photolithography exposes the hydrophilic areas to polymers (photoresists) and solvents, not only reducing the hydrophilicity, but also affecting the TMB-H2O2 assay system with an unavoidable incomplete elimination of photoresist during fabrication. Detection signals are then observed in the presence of photoresist residues on the P-PAD, even at a blank HRP concentration.日本分析化学会Journal Articleapplication/pdfhttp://hdl.handle.net/2115/63206https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/63206/1/Tokeshi-AS32%288%29.pdf0910-6340Analytical sciences3288158182016-08enginfo:pmid/27506705info:doi/10.2116/analsci.32.815publisher