2024-03-29T14:47:38Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/643942022-11-17T02:08:08Zhdl_2115_20039hdl_2115_116Specificity of Chroomonas (Cryptophyceae) as a source of kleptochloroplast for Nusuttodinium aeruginosum (Dinophyceae)Onuma, RyoHoriguchi, TakeoChroomonaskleptochloroplastNusuttodinium aeruginosumprey specificitysingle cell PCR400The unarmoured dinoflagellate Nusuttodinium aeruginosum retains a kleptochloroplast, which is a transient chloroplast stolen from members of the cryptomonad genus, Chroomonas. Both N.aeruginosum and the closely related N.acidotum have been shown to restrict their diet to a limited number of species of this blue-green genus of cryptophyte. However, it is still unclear how flexible the predators are with regard to the ingestion and utilization of Chroomonas spp. as a source of kleptochloroplast. To address specificity of cryptomonad in N.aeruginosum, we collected the cells of N.aeruginosum from several ponds in Japan, and analysed the phylogeny of the kleptochloroplasts based on their plastidial 16S rDNA sequences. All sequences obtained in this study were restricted to only one (the subclade 4) of four subclades known to comprise the Chroomonas/Hemiselmis clade. Therefore, N.aeruginosum is specific in its dietary requirements, selecting their prey within the subclade level.Wiley-BlackwellJournal Articleapplication/pdfhttp://hdl.handle.net/2115/64394https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/64394/1/MS.pdf1322-0829Phycological research64135432016-01enginfo:doi/10.1111/pre.12117This is the peer reviewed version of the following article: Phycological research, 64(1), 2016, pp35-43, which has been published in final form at 10.1111/pre.12117. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.author