2024-03-28T11:10:11Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/677712022-11-17T02:08:08Zhdl_2115_20049hdl_2115_141Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl-gamma-cyclodextrin-modified micellar electrokinetic chromatographyKodama, ShujiNakajima, ShotaOzaki, HiromichiTakemoto, RyotaItabashi, YutakaKuksis, ArnisEnantioseparationGracilariaHydroxyeicosatetraenoic acidHydroxypropyl-gamma-cyclodextrinMicellar electrokinetic chromatography660Complete resolution of hydroxyeicosatetraenoic acid (HETE) enantiomers was achieved using hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD)-modified MEKC. The optimum running conditions were determined to be utilizing a 30 mM phosphate-15 mM borate buffer (pH 9.0) containing 30 mM HP-gamma-CD and 75 mM SDS as the BGE, application of +30 kV as the effective voltage, and carrying out the experiment at 15 degrees C. The eluents were detected at 235 nm. The method was used successfully for the simultaneous separations of (S)- and (R)-enantiomers of regioisomeric 8-, 11-, 12-, and 15-HETEs. Subsequently, the optimized method was applied to evaluate the stereochemistry of 8- and 12-HETEs from the marine red algae, Gracilaria vermiculophylla and Gracilaria arcuata, respectively. The 8-HETE was found to be a mixture of 98% (R)-enantiomer and 2% (S)-enantiomer, while the 12-HETE was a mixture of 98% (S)-enantiomer and 2% (R)-enantiomer. The present study demonstrates that the HP-gamma-CD-modified MEKC method is simple and sensitive and provides unambiguous information on the configuration of natural and synthetic HETEs.Wiley-BlackwellJournal Articleapplication/pdfhttp://hdl.handle.net/2115/67771https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/67771/1/Text%20%282017.03.08%29%20for%20HUSCAP.pdf0173-08351522-2683AA11619422Electrophoresis3723-24319632052016-12enginfo:pmid/27649837info:doi/10.1002/elps.201600213This is the peer reviewed version of the following article:ELECTROPHORESIS December 2016,37(23-24), p.3196-3205, which has been published in final form at 10.1002/elps.201600213. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.author