2024-03-29T01:00:10Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/683452022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138The localization of GATA2 in the nuclear and cytoplasmic regions of ovine conceptusesBai, HanakoSakurai, ToshihiroBai, RulanGodkin, James D.Imakawa, KazuhikoGATA2localizationovine conceptuses460Abstract:GATA transcription factors are emerging as critical regulators in trophoblast development and its gene regulation. The purpose of this study was to examine the expression and cellular localization of GATA2 in ovine conceptuses during the peri-implantation period. In Western blot analyses, GATA2 proteins were found in days 15, 17 and 21 ovine conceptuses (day 0 = day of estrus). Using immunohistochemistry and immunofluorescence analyses, we found that GATA2 was localized in days 15, 17 and 21 ovine conceptuses, and more importantly, GATA2 protein was detected in both nuclear and cytoplasmic regions of the trophectoderm. To our knowledge, the present study is the first to demonstrate that GATA2 is localized in two cellular compartments of the trophectoderm in ovine and many other mammalian species, and suggests that the difference in GATA2 location plays a role in the regulation of down-stream genes during the early pregnancy period.Journal Articleapplication/pdfhttp://hdl.handle.net/2115/68345https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/68345/1/5.%20Bai_Anim.Aci.J..pdf1344-3941Animal Science Journal85129819852014-12enginfo:doi/10.1111/asj.12267© 2014 Japanese Society of Animal Science., This is the peer reviewed version of the following article: [Animal Science Journal; 85(12): pp981-985, 2014], which has been published in final form at [10.1111/asj.12267]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.author