2024-03-29T08:23:45Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/684262023-08-15T04:57:47Zhdl_2115_20042hdl_2115_136Activation of nucleotide-binding domain-like receptor containing protein 3 inflammasome in dendritic cells and macrophages by Streptococcus sanguinisSaeki, AyumiSuzuki, ToshihikoHasebe, AkiraKamezaki, RyousukeFujita, MariNakazawa, FutoshiShibata, Ken-Ichiro497Streptococcus sanguinis is frequently isolated from the blood of patients with infective endocarditis and contributes to the pathology of this disease through induction of interleukin (IL)-1β responsible for the development of the disease. However, the mechanism of IL-1β induction remains unknown. In this study, S. sanguinis activated a murine dendritic cell (DC) to induce IL-1β and this activity was attenuated by silencing the mRNAs of nucleotide-binding domain-like receptor containing protein 3 (NLRP3) and caspase-1. S. sanguinis induced IL-1β production in murine bone marrow-derived macrophage (BMM), but this activity was significantly reduced in BMMs from NLRP3-, apoptosis-associated speck-like protein containing a caspase-recruitment domain-, and caspase-1-deficient mice. DC phagocytosed S. sanguinis cells, followed by the release of adenosine triphosphate (ATP). The ATP-degradating enzyme attenuated the release of ATP and IL-1β. The inhibitors for ATP receptor reduced IL-1β release in DC. These results strongly suggest that S. sanguinis has the activity to induce IL-1β through the NLRP3 inflammasome in macrophage and DC and interaction of purinergic receptors with ATP released is involved in expression of the activity.Wiley-BlackwellJournal Articleapplication/pdfhttp://hdl.handle.net/2115/68426https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/68426/1/CM%20S%20sanguinis%202nd%20revised.pdf1462-58141462-5822Cellular Microbiology193e126632017-03enginfo:pmid/27601185info:doi/10.1111/cmi.12663This is the peer reviewed version of the following article: [Activation of nucleotide-binding domain-like receptor containing protein 3 inflammasome in dendritic cells and macrophages by Streptococcus sanguinis.], which has been published in final form at [http://doi.org/10.1111/cmi.12663]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.author