2024-03-28T21:05:42Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/686542022-11-17T02:08:08Zhdl_2115_20044hdl_2115_124Fructose suppresses uric acid excretion to the intestinal lumen as a result of the induction of oxidative stress by NADPH oxidase activationKaneko, ChihiroOgura, JiroSasaki, ShunichiOkamoto, KeisukeKobayashi, MasakiKuwayama, KaoriNarumi, KatsuyaIseki, KenHyperuricemiaFructoseUric acid excretionBreast cancer resistance proteinOxidative stressNADPH oxidase460Background: A high intake of fructose increases the risk for hyperuricemia. It has been reported that long-term fructose consumption suppressed renal uric acid excretion and increased serum uric acid level. However, the effect of single administration of fructose on excretion of uric acid has not been clarified. Methods: We used male Wistar rats, which were orally administered fructose (5 g/kg). Those rats were used in each experiment at 12 h after administration. Results: Single administration of fructose suppressed the function of ileal uric acid excretion and had no effect on the function of renal uric acid excretion. Breast cancer resistance protein (BCRP) predominantly contributes to intestinal excretion of uric acid as an active homodimer. Single administration of fructose decreased BCRP homodimer level in the ileum. Moreover, diphenyleneiodonium (DPI), an inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox), recovered the suppression of the function of ileal uric acid excretion and the Bcrp homodimer level in the ileum of rats that received single administration of fructose. Conclusions: Single administration of fructose decreases in BCRP homodimer level, resulting in the suppression the function of ileal uric acid excretion. The suppression of the function of ileal uric acid excretion by single administration of fructose is caused by the activation of Nox. The results of our study provide a new insight into the mechanism of fructose-induced hyperuricemia. (C) 2016 Elsevier B.V. All rights reserved.ElsevierJournal Articleapplication/pdfhttp://hdl.handle.net/2115/68654https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/68654/1/WoS_77643_kobayashi.pdf0304-4165Biochimica et Biophysica Acta (BBA) General subjects186135595662017-03enginfo:pmid/27913188info:doi/10.1016/j.bbagen.2016.11.042© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/author