2024-03-29T14:31:02Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/706902022-11-17T02:08:08Zhdl_2115_35410hdl_2115_35409Photo-Activatable Akt Probe: A New Tool to Study the Akt-Dependent Physiopathology of Cancer CellsHaga, SanaeOzawa, TakeakiMorita, NaokiAsano, MamiJin, ShigekiYiminOzaki, MichitakaPhoto-activatable probeAktNutritional deprivationOxidative stress490Akt is commonly overexpressed and activated in cancer cells and plays a pivotal role in cell survival, protection, and chemoresistance. Therefore, Akt is one of the target molecules in understanding characters of cancer cells and developing anticancer drugs. Here we examined whether a newly developed photo-activatable Akt (PA-Akt) probe, based on a light-inducible protein interaction module of plant cryptochrome2 (CRY2) and cryptochrome-interacting basic helix-loop-helix (CIB1), can regulate Akt-associated cell functions. By illuminating blue light to the cells stably transfected with PA-Akt probe, CRY2-Akt (a fusion protein of CRY2 and Akt) underwent a structural change and interacted with Myr-CIBN (myristoylated N-terminal portion of CIB1), anchoring it at the cell membrane. Western blot analysis revealed that 5473 and T308 of the Akt of probe-Akt were sequentially phosphorylated by intermittent and continuous light illumination. Endogenous Akt and GSK-3 beta, one of the main downstream signals of Akt, were also phosphorylated, depending on light intensity. These facts indicate that photo-activation of probe-Akt can activate endogenous Akt and its downstream signals. The photo-activated Akt conferred protection against nutritional deprivation and H2O2 stresses to the cells significantly. Using the newly developed PA-Akt probe. endogenous Akt was activated easily, transiently, and repeatedly. This probe will be a unique tool in studying Akt-associated specific cellular functions in cancer cells and developing anticancer drugs.Cognizant Communication CorporationJournal Articleapplication/pdfhttp://hdl.handle.net/2115/70690https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/70690/1/s13.pdf0965-04071555-3906AA10833653Oncology research2634674722018-04-10enginfo:pmid/28933316info:doi/10.3727/096504017X15040166233313http://creativecommons.org/licenses/by-nc/3.0/publisher