2024-03-28T12:15:06Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/710282022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138Exosomes isolated from sera of mice fed Lactobacillus strains affect inflammatory cytokine production in macrophages in vitroAoki-Yoshida, AyakoSaito, ShinichiTsuruta, TakeshiOhsumi, ArisaTsunoda, HinakoSonoyama, KeiProbioticsInflammationTNF-alphaIL-6Exosome464Orally administered Lactobacillus strains, including L. plantarum No.14 and L rhamnosus GG, reportedly reduce inflammatory cytokine production in mice. The present study tested our idea that circulating exosomes mediate the action of Lactobacillus strains. The lipopolysaccharide-induced production of TNF-alpha and IL-6 in vitro was attenuated in peritoneal exudate cells (PECs) isolated from C57BL/6N mice that had been fed L. plantarum No.14. When PECs were cultured for 24 h with exosomes isolated from the serum of mice fed L plantarum No.14 or L. rhamnosus GG, accumulation of both TNF-alpha and of the corresponding mRNA was lowered. Growth in the presence of these exosomes also decreased the production of TNF-alpha and IL-6 by the murine macrophage cell line RAW264.7. In contrast, supplementation with exosome-depleted serum of mice fed L plantarum No.14 or L. rhamnosus GG failed to affect the production of TNF-alpha and IL-6 by RAW264.7 cells. When PECs and RAW264.7 cells were cultured for 24 h with PKH67-labeled exosomes isolated from murine serum, fluorescent signal was observed inside the cells, suggesting that these cells incorporate serum exosomes. We propose that the anti-inflammatory activity of orally administered L plantarum No.14 and L rhamnosus GG is mediated, at least in part, by circulating exosomes.ElsevierJournal Articleapplication/pdfapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheethttp://hdl.handle.net/2115/71028https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/71028/1/Aoki2017BBRC.pdfhttps://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/71028/2/mmc1.xlsxhttps://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/71028/3/mmc2.xlsx0006-291X1090-2104AA00564395Biochemical and biophysical research communications48922482542017-07-22enginfo:pmid/28559134info:doi/10.1016/j.bbrc.2017.05.152(C) 2017 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/author